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15-PGDH Inhibitor Screening Kit (Fluorometric)

Simple assay to screen/study/characterize COX-2 Inhibitors
Catalog #: K503
$625.00

Product Details

Cat # +Size K503-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 535/587 nm)
Applications Screening/studying/characterizing 15-PGDH inhibitors.
Features & Benefits • Simple method to screen 15-PGDH inhibitors
• High-throughput Compatible
• Includes 15-PGDH Inhibitor Control
Kit Components • 15-PGDH Assay Buffer
• PicoProbe™ Probe (in DMSO)
• 15-PGDH Developer
• 15-PGDH Substrate (in DMSO)
• 15-PGDH Enzyme
• 15-PGDH Inhibitor Control (in DMSO)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

15-hydroxyprostaglandin dehydrogenase (15-PGDH, EC: 1.1.1.141) catalyzes the oxidation of Prostaglandins (PG) to 15-keto metabolites which show greatly reduced biological activity when compared to Prostaglandins. 15-PGDH catalyzes the rate-limiting step of PG catabolism. More recently, the inhibition of 15-PGDH has been reported as a negative regulator of tissue repair and inhibition of this enzyme can potentiate tissue regeneration in mouse models. Thus, pharmacological inhibition of 15-PGDH could have clinical implications by accelerating potential tissue regeneration after damage. In this assay, 15-PG is oxidized by 15-PDGH generating 15-Keto metabolites and oxidizing NAD. NADH reduces a Fluorometric probe thus, generating an enhanced and stable fluorescence signal (Ex/Em=535/587 nm). In the presence of an inhibitor, the enzymatic activity is inhibited resulting No/Low fluorescence. BioVision’s 15-PGDH Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of 15-PGDH inhibitors.


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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
 BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.