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11-Dehydrothromboxane B2 ELISA Kit

For in vitro quantitative determination of 11dhTxB2Highly Sensitive, IHC/ICC Kit

WARNING: This product can expose you to chemicals including Dimethylformamide (DMF), which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E4900
$545.00

Product Details

Cat # +Size E4900-100
Size 100 assays
Kit Summary BioVision’s 11-Dehydrothromboxane B2 ELISA Kit can quickly and reliably determine a broad range of 11dhTxB2 concentrations in human urine samples. It is a competitive ELISA, based on the competition between the free 11dhTxB2 and 11dhTxB2-Acethylcholinesterase (AChE) conjugate for a constant amount of 11dhTxB2 monoclonal antibody binding sites. Thus, in the presence of an AChE substrate, the absorbance of the released product is inversely proportional to the amount of 11dhTxB2 in samples. The kit can detect as low as 15.6 pg/ml of 11dhTxB2 in 40 minutes.
Detection Method Absorbance at 412 nm
Species Reactivity Human
Applications For in vitro quantitative determination of 11dhTxB2 in human urine samples.
Features & Benefits • A competitive ELISA based kit
• A quick and reliable method
• For in vitro quantitative determination of 11dhTxB2 in human urine samples
• The kit can detect as low as 15.6 pg/ml of 11dhTxB2 in 60 minutes
Kit Components • Pre-coated 96 well strip plate
• 11dhTxB2 Standard
• 11dhTxB2-AChE
• 11dhTxB2 Monoclonal Antibody
• 11dhTxB2 Incubation Buffer (10X)
• AChE Substrate
• AChE Probe
• 11dhTxB2 Developer
• Wash Buffer (10X)
• Plate Sealers
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

11-Dehydrothromboxane B2 (11dhTxB2 or 11-dehydro-TxB2) is a stable metabolite of Thromboxane A2 (TxA2). TxA2 is a prothrombotic agent capable of promoting the activation and subsequent aggregation of platelets nearby. It is derived from Arachidonic Acid in the COX pathway and is involved in maintaining hemostasis & preventing cardiovascular diseases. TxA2 has a very short half-life and is quickly hydrolyzed to thromboxane B2 (TxB2), which further metabolizes to 11dhTxB2. 11dhTxB2 has a stable half-life and is excreted in the urine. It is unaffected by the ex vivo platelet activation and other pre-analytical variables. Urinary 11dhTxB2 can directly reflect the platelet production of TxA2 and represents a good and reliable biomarker for platelet activity. Additionally, Aspirin can irreversibly inhibit the platelet COX-1 activity thereby resulting in the inhibition of TxA2 production and thromboxane-mediated platelet activation. Thus, urine levels of 11dhTxB2 reflect COX-1 inhibition and can be used to monitor responses to Aspirin therapy. BioVision’s 11-Dehydrothromboxane B2 ELISA Kit can quickly and reliably determine a broad range of 11dhTxB2 concentrations in human urine samples. It is a competitive ELISA, based on the competition between the free 11dhTxB2 and 11dhTxB2-Acethylcholinesterase (AChE) conjugate for a constant amount of 11dhTxB2 monoclonal antibody binding sites. Thus, in the presence of an AChE substrate, the absorbance of the released product is inversely proportional to the amount of 11dhTxB2 in samples. The kit can detect as low as 15.6 pg/ml of 11dhTxB2 in 60 minutes.


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