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1,5-Anhydroglucitol Uptake Assay Kit (Cell-Based)

Non-radioactive method to detect and accurately measure 1,5-Anhydroglucitol uptake via FACS and microscopy
Catalog #: K684
$875.00

Product Details

Cat # +Size K684-50
Size 50 assays
Detection Method FACS (488 nm excitation laser) and fluorescent microscope (excitation range 420 nm-495 nm)
Applications Measurement of 1,5-Anhydroglucitol uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc.
Dual-staining of 1,5-AG transporters and 1,5-AG uptake
Analysis of 1,5-AG metabolism and cell signaling in various cell types
Screening of anti-diabetic compounds
Features & Benefits • Easy-to-use
• Non-radioactive
• Image and accurately measure 1,5-Anhydroglucitol uptake in cultured cells in response to insulin, growth factors etc.
Kit Components • Analysis Buffer
• AGTracker™ Reagent (100X)
• Phloretin (100X) (in DMSO)
• Phloretin (100X)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

1,5-Anhydroglucitol (1,5-AG) is the 1-deoxy form of D-glucose. Its level in blood plasma has been used in clinical research to investigate short-term glycemic control levels in diabetic patients. Cereals and animal source proteins constitute the main 1,5-AG source for humans. Most of 1,5-AG is absorbed by renal tubules, and its levels in human body are highly regulated and relatively consistent. Glucose can act as a competitive inhibitor of 1,5-AG in blood. Therefore, glucose levels are inversely proportional to 1,5-AG concentrations. The physiological function of 1,5-AG is not well understood. Recent studies showed 1,5-AG uptake could be associated with sodium-dependent glucose transporter. BioVision’s 1,5-Anhydroglucitol Uptake Assay Kit utilizes a proprietary fluorescent 1,5-AG analog, which can be taken up by cells. However, this 1,5-AG analog cannot be fully utilized in glycolytic processes and thus accumulates inside the cells. Fluorescence generated by this analog is proportional to the cellular 1,5-AG uptake. The assay can be used to monitor 1,5-AG uptake by using fluorescent microscopy and flow cytometry. Phloretin, a compound that can inhibit glucose transporter and 1,5-AG cell uptake, is included in the kit as a control. This non-radioactive assay kit is easy-to-use and allows qualitative and quantitative measurements of 1,5-AG uptake in cultured cells


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Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.