Triglyceride Assay Kit from BioVision. Triglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis. We have developed the Triglyceride Assay Kit, an easy and sensitive assay to measure triglyceride concentration in variety of samples. In the assay, triglycerides are converted to free fatty acids and glycerol. Then the glycerol is oxidized to a product which reacts with our probe to form a product that is easily quantified by either colorimetric (spectrophotometry at = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect 0.1-10nmol (or 2~10000mM range) of glycerol in various samples.
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