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Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue. The NAD/NADH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NAD, NADH and their ratio. The enzymes in the system specifically recognize NAD/NADH in an enzyme cycling reaction. (It does not detect NADP/NADPH). There is no need to purify NAD/NADH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity.
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Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue. The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NAD+/NADH). There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Results can be quantified using plate reader at OD450 nm.
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