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The regulation of HDL (high-density-lipoprotein)-cholesterol and LDL (low-density-lipoprotein)-cholesterol plays a central role in various disease development. It is well known that low levels of HDL and high level of LDL are associated with an increased risk of cardiovascular events. The HDL and LDL/VLDL Cholesterol Quantification Kit provides a simple method for convenient separation of HDL from LDL and VLDL (very low-density lipoprotein) in serum sample, then HDL and LDL/VLDL cholesterol levels can easily be quantified separately, using cholesterol oxidase mediated reactions. Cholesterol esterase hydrolizes cholesteryl ester into free cholesterol, which can then be oxidized by cholesterol oxidase to yield H2O2. The H2O2 interacts with a sensitive cholesterol probe to produce resorufin, which can be detected with a micro-plate reader at l = 570 nm or fluorescence micro-plate reader at Ex/Em = 538/587 nm. The assay can also detect cholesterol ester and free cholesterol separately in these two fractions by adding or not adding cholesterol esterase into the reactions.
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