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Choline and acetylcholine play important roles in many biological processes. BioVision’s Choline, Acetylcholine Quantification Kit provides a simple and sensitive tool to quantify Choline or Acetylcholine by either a colorimetric or fluorometric method. In the kit, free choline is oxidized to betaine, through the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate color (l= 570 nm), and fluorescence (Ex/Em 535/587 nm). Acetylcholine can be converted to choline by the addition of acetylcholinesterase in the reaction. The kit can detect choline, acetylcholine (total choline – free choline) in biological samples such as in blood, cells, culture media, fermentation media, etc. There is no need for pretreatment or purification of samples. The kit can detect 10 pmol~5 nmol of choline or acetylcholine.
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