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Glutathione Peroxidase Assay Kit. Glutathione Peroxidase (GPx, EC 1.11.1.9) is an enzyme family with peroxidase activity, and plays important role in protecting of organisms from oxidative damage. It converts reduced glutathione (GSH) to oxidized glutathione (GSSG), to reduce lipid hydroperoxides to their corresponding alcohols, or reduce free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders. BioVision’s Glutathione Peroxidase Assay Kit measures glutathione peroxidase (GPx) activity through a coupled reaction with glutathione reductase (GR). In the assay, GPx reduce Cumene Hydroperoxide, and oxidize GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by GR. The decrease of NADPH is proportionally to GPx activity in the reactions. The decrease of NADPH can be easily measured by absorbance at 340 nm. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with detection sensitivity ~ 0.5 mU/ml of GPx in samples.
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