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Coenzyme A Assay Kit. Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main function of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates. BioVision has developed an easy, convenient assay to measure the CoA level in variety biological samples. In the assay, CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (λ = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 uM concentration range) in a variety of samples.
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