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Activation of protein kinase, such as JNK and Akt, plays an important role in apoptosis and other physiological and pathological processes. BioVision provides a novel nonradioactive system for convenient measurement of JNK and Akt activities. The JNK Activity Screening Kit is used to screen JNK activity relatively quickly for large numbers of samples. The assay utilizes a N-terminal c-Jun fusion protein bead to selectively “pull down” JNK from cell lysate. c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody. The KinaseSTARTM JNK or Akt Activity Assay Kit utilizes a JNK- (or Akt-) specific antibody to immunoprecipitate JNK (or Akt) from cell lysate. JNK- (or Akt-) specific activity is then analyzed by Western blot analysis of the phosphorylation levels of c-Jun (or GSK-3a) using a phospho-c-Jun (or phospho-GSK-3a) specific antibody. The kit specifically detects JNK (or Akt) activity, other kinase activities would not be detected.
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Analyses of JNK Specific Activity in Jurkat Extracts. Jurkat cells were untreated (Lane 3), or treated with anisomycin (10 ug/ml, Lane 1) or NaCl (0.5 M, Lane 2) for 30 min. Cell lysates were immunoprecipitated using a JNK-specific polyclonal antibody. JNK specific activity was then analyzed by Western blot analysis of the phosphorylation levels of c-Jun (1-79), using a phospho-c-Jun specific antibody included in the kit.
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| Kinase Activity Assay Kits | |||||
| Name | Cat. # | Size | Price (USD) | Data Sheet  |
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| KinaseSTARTM Akt Activity Assay Kit | K435-40 | 40 assays | $495.00 | Product Data Sheet |  |
| KinaseSTARTM JNK Activity Screening Kit | K430-40 | 40 assays | $395.00 | Product Data Sheet | |
| KinaseSTARTM JNK Activity Assay Kit | K431-40 | 40 assays | $495.00 | Product Data Sheet | |
| Kinase Substrates | |||||
| Name | Cat. # | Size | Price (USD) | Data Sheet |
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| c-Jun (1-79) Fusion Protein | 7001-100 | 100 ug | $105.00 | Product Data Sheet | |
| GSK-3a Fusion Protein | 7003-100 | 100 ug | $105.00 | Product Data Sheet | |
| JNK Activators | |||||
| Name | Cat. # | Size | Price (USD) | Data Sheet |
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| Anisomycin | 1549-10 | 10 mg | $95.00 | Product Data Sheet | |
| TNF-a, human, recombinant | 1050-10 | 10 ug | $75.00 | Product Data Sheet | |
| TNF-a, human, recombinant | 1050-50 | 50 ug | $180.00 | Product Data Sheet | |
| TNF-a, mouse, recombinant | 1051-10 | 10 ug | $125.00 | Product Data Sheet | |
| TNF-a, rat, recombinant | 1052-10 | 10 ug | $125.00 | Product Data Sheet | |
| TNF-a, rat, recombinant | 1052-50 | 50 ug | $295.00 | Product Data Sheet | |
| TNF-a, rat, recombinant | 1052-1000 | 1 mg | $2950.00 | Product Data Sheet | |
| Kinase and Related Inhibitors | |||||
| Name | Cat. # | Size | Price (USD) | Data Sheet |
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| L-JNKI1 | 7011-50 | 50 ul (1 mM) | $135.00 | Product Data Sheet | |
| [L-Form JNK Peptide Inhibitor 1, ready-to-use at 1000X dilution] | |||||
| L-JNKI1 Control Peptide | 7021-50 | 50 ul (1 mM) | $95.00 | Product Data Sheet | |
| [Negative Control Peptide for L-JNKI1] | |||||
| Akt Inhibitor | 1701-1 | 1 mg | $135.00 | Product Data Sheet | |
| (1L-6-Hydroxymethyl-Chiroinositol2-[(R)-2-O-methyl-3-Ooctadecylcarbonate]) | |||||
| Chk2 Inhibitor | 1702-1 | 1 mg | $105.00 | Product Data Sheet | |
| (2-(4-(4-Chlorophenoxy)phenyl)-1H-benzimidazole-5-carboxamide) | |||||
| Proteasome Inhibitor | 1703-5 | 5 mg | $99.00 | Product Data Sheet | |
| (MG-132, Z-LLL-CHO) | |||||
| JNK & Akt Control Cell Lysates | |||||
| Name | Cat. # | Size | Price (USD) | Data Sheet |
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| JNK Negative Control Cell Lysate | 7031-1 | 1 mg | $225.00 | Product Data Sheet | |
| JNK Activated Cell Lysate | 7032-1 | 1 mg | $225.00 | Product Data Sheet | |
| Akt Negative Control Cell Lysate | 7035-1 | 1 mg | $225.00 | Product Data Sheet | |
| Akt Activated Cell Lysate | 7036-1 | 1 mg | $225.00 | Product Data Sheet | |
| Akt & JNK Antibodies | Cat. # | Host | Species | Application | Size | Price (USD) | Data Sheet |
Add to Cart |
| Akt/PKB | 3247-100 | Rb | hu/ms/rt/bv | WB/IP/IHC | 100 ug | $ | Product Data Sheet | |
| Phospho-Akt | 3257-100 | Rb | hu/ms/rt | WB/IP | 100 ug | $ | Product Data Sheet | |
| Phospho-JNK/SAPK | 3589-100 | Rb | hu/ms/rt | WB/IP/IHC/ICC | 100 ug | $ | Product Data Sheet | |
| JNK | 3701-100 | Rb | hu/ms/rt | WB/IP/IH | 100 ug | $ | Product Data Sheet | |
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