Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.
Standard Calibration Curve of GST Measured by spectrophotometry. Various amounts of standard GST was incubated with GSH and CDNB according to the kit instructions. Absorbances were measured at 340 nm.
Inhibition of GST Activities by GST Inhibitors. Various amounts of GST Inhibitor-1 (Cat. 1555-1000) or GST Inhibitor-2 (Cat. 1556-1000) were added to GST assay reactions. GST activity was measured by GST Fluorometric Assay Kit (Cat. K260-100) according to the kit instructions.
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