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Glutathione is the major intracellular low-molecular-weight thiol. It plays a critical role in cellular defense against oxidative stress in tissues and cells. BioVision offers three sensitive, easy-to-use glutathione detection kits. The Glutathione Fluorometric Detection Kit (K251-100) utilizes monochlorobimane (MCB), a dye that has a high affinity for glutathione, to measure the change of glutathione level during pathological processes (e.g., apoptosis). The Glutathione Colorimetric Detection Kit (K261-100) utilizes the glutathione recycling system by DTNB and glutathione reductase to quantify total glutathione and the reduced form of glutathione. The recycling system dramatically improves the detection sensitivity. The novel Oxidized, Reduced, and Total Glutathione Detection Kit (K264-100) utilizes special formulations of buffers and reagents to quantify the oxidized, reduced, and total glutathione separately. This is the only commercially available kit that can detect oxidized and reduced glutathione individually.Glutathione Reductase Assay Kit (K761-200) is also available.
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| Glutathione Fluorometric Detection Kit |
K251-100 |
100 assays |
$305.00 |
Product Data Sheet |
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The kit provides a fluorescence method to detect the change in glutathione level in apoptotic cells or other pathological processes using a fluorometer or a 96-well fluorometric plate reader (Ex/Em = 380/460 nm).
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| Glutathione Assay Kit (GSH, GSSG and Total) |
K264-100 |
100 assays |
$385.00 |
Product Data Sheet |
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The novel glutathione assay kit detects the GSH, GSSG, and total glutathione individually using a fluorescence method (Ex/Em = 340/450 nm) in 96 well microtiter plates. The kit also provides unique procedure and buffer formula to eliminate protein thiol interference and thus stabilize GSH and GSSG in solutions. The assay is easy to perform and detects 2-400 ng/ml of GSH, GSSG or total glutathione.
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| Glutathione Reductase Assay Kit |
K761-200 |
200 assays |
$295.00 |
Product Data Sheet |
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| This Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5′-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, -max = 405 nm). The assay can detect 0.1 – 40 mU/ml GR in various samples. |
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| Glutathione Peroxidase Assay Kit |
K762-100 |
100 assays |
$295.00 |
Product Data Sheet |
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| Glutathione Peroxidase Assay Kit measures glutathione peroxidase (GPx) activity through a coupled reaction with glutathione reductase (GR). In the assay, GPx reduce Cumene Hydroperoxide, and oxidize GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by GR. The decrease of NADPH is proportionally to GPx activity in the reactions. The decrease of NADPH can be easily measured by absorbance at 340 nm. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with detection sensitivity ~ 0.5 mU/ml of GPx in samples. |
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| Thioredoxin Reductase Assay Kit |
K763-100 |
100 assays |
$295.00 |
Product Data Sheet |
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| BioVision’s Thioredoxin Reductase Assay Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the assay TrxR catalyzes the reduction of 5, 5’-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generate a strong yellow color (λmax = 405 nm). |
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K251-100 GSH Level Decreases Following Induction of Apoptosis. Jurkat cells were treated with camptothecin for 8 hours. GSH level was measured using the ApoGSHTM Fluorometric kit according to kit instructions.
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K264-100 Glutathione assays were prepared using standard GSH and GSSG following the kit instructions. Diamond (blue) represents GSH standard curve. Square (pink) represents standard curve produced by adding GSSG + GSH Quencher + Reducing Agent Mix. Triangle (gray) represents GSS92G standard without adding Reducing Agent mix.
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K261-100 Glutathione Standard Calibration Curve.
10 minutes incubation at room temperature.
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= 405 or 415 nm). The recycling system utilized in the assay dramatically improves the detection sensitivity.