Cholesteryl ester transfer protein (CETP) is a member of the lipid transfer/lipopolysaccharide binding protein gene family. CETP transfers neutral lipids from high density lipoprotein (HDL) to very low density lipoprotein (VLDL) and is present in normal human plasma and serum. CETP plays a major role in the catalism of HDL and, therefore, influences the concentration, apolipoprotein content, and size of HDL particles in plasma. Genetic deficiency in CETP results in a variety of abnormalities in the concentration, composition and function of high density and low density lipoproteins. CETP deficiency is also associated with an increased risk for colonary heart disease, despite a parallel increase in HDL levels. The CETP Activity Assay Kit uses a donor molecule containing a fluorescent neutral lipid that is transferred to an acceptor molecule in the presence of CETP from a given plasma and serum sample. The fluorescent neutral lipid is present in a self-quenched state when contained within the core of the donor molecule. CETP-mediated transfer of the fluorescent neutral lipid to the acceptor molecule results in an increase in fluorescence (Excitation: 465; Emission: 535).
|
(K601-100) Published References:
|
|
1) Hime NJ et al (2008) J. Lipid Res. 49: 2113 - 2123.
2) Leorard SW et al. (2007) Am. J. Health Syst. Pharm. 64: 2257-2266.
3) Satoh N. et al. (2007) Diabetes Care 30: 144-146.
4) Shrestha S et al. (2007) J. Nutr. 137: 1165-1170.
|
|
|
|
(K602-100) Published References:
|
|
1) Hime NJ et al (2008) J. Lipid Res.; 49: 2113 - 2123.
2) Leorard SW et al. (2007) Am. J. Health Syst. Pharm. 64: 2257-2266.
3) Satoh N. et al. (2007) Diabetes Care 30: 144-146.
4) Shrestha S et al. (2007) J. Nutr. 137: 1165-1170.
|
|
|
Plasma phospholipid transfer protein (PLTP) is thought to play a major role in the facilitated transfer of phospholipids between lipoproteins and in the modulation of high-density lipoprotein (HDL) particle size and composition. PLTP-facilitated lipid transfer activity is related to HDL and LDL metabolism, as well as lipoprotein lipase activity, adiposity, and insulin resistance. The PLTP Activity Assay Kit uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of PLTP. PLTP-mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Excitation: 465 nm; Emission: 535 nm).
|
|
|
