CaspSCREEN Flow Cytometric Apoptosis Detection Kit :: 800-891-9699

20 minutes procedure · Highly sensitive · Screen caspase activity by flow cytometry

This CaspSCREEN^TM Apoptosis Assay Kit provides a convenient means for detecting the activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D2R), a reported substrate for members of caspase family proteases. The caspase substrate D2R is nonfluorescent, however, upon cleavage of the substrate by cellular activated caspases, the released rhodamine 110 gives rise to fluorescence that can be measured by flow cytometry at excitation of 488 nm and emission of 530 nm. The kit includes the D2R reagent, DTT and the optimal incubation buffer together with an application protocol.

Detection of Caspase-3 Activation Using CaspGLOWTM Fluorescein Caspase-3 Staining Kit. Apoptosis was induced in Jurkat cells with (B) or without (A) camptothecin for 6 hours. Cells were collected and incubated with CaspGLOWTM in situ marker, FITC-DEVD-FMK, for 20 minutes according to the kit instructions. Results were analyzed by flow cytometry.

Product	Cat. #	Size	Price (USD)	Data Sheet	Add to Cart
CaspSCREEN^TM Flow Cytometric Apoptosis Detection Kit	K200-25	25 assays	$165.00	Product Data Sheet
CaspSCREEN^TM Flow Cytometric Apoptosis Detection Kit	K200-100	100 assays	$365.00	Product Data Sheet

For more information: BioVision, Inc. :: 980 Linda Vista Avenue :: Mountain View, CA 94043 US
Email: tech@biovision.com :: 650-428-0236 :: 1-800-891-9699 :: Fax: 650-428-0336
Business Hours: M-F 7:00 A.M.-5:00 P.M. (Pacific Standard Time)

© copyright 2008 BioVision, Inc. All Rights Reserved.