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CaspGLOW In Situ Caspase Staining Kits provide simple and sensitive tools for detecting activated caspases in situ in living cells. The assay utilizes potent caspase inhibitors that were conjugated to FITC or rhodamine as the fluorescence in situ markers. The caspase inhibitors are cell permeable, nontoxic, and irreversibly bind to the activated caspases in apoptotic cells. With the FITC or rhodamine labels, activated caspases in apoptotic cells can directly be visualized by fluorescence microscopy, or analyzed by flow cytometry or fluorescence plate reader. Caspase inhibitors without FITC or rhodamine labels were also included in the kits as negative controls.
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