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Calpain, a cystolic calcium-dependant cysteine protease, is invovled in many foms of apoptosis. The Calpain Activity Assay Kit provides a simple and convenient means for analyzing the activated calpain activity in apoptotic and other samples The assays is based on fluorometric detection of the cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFCemits blue light (lmax=400nm); upon cleavage of the substrate by calpain or related proteases, free AFC emits a yellow-green fluorescence (lmax=505nm), which can be quantified using fluorometer or a fluorescence plate reader. Comparison of the fluorescence of AFC from a treated sample with a normal control allows determination of the fold increase in calpain activity. The proprietary Calpain Extraction Buffer provided in the kit specifically extracts activated calpain from cytosol, but not other proteasesin lysosomes. The kit also provides active calpain as the positive control and calpain inhibitor as the negative control. Active calpain, calpain inhibitor,and anti-calpain antibody are also available separately.
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(K240-100) Published References:
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| Assay Kit | Cat. # | Size | Price (USD) | Data Sheet  |
Add to Cart |
| Calpain Activity Assay Kit | K240-100 | 100 assays | $355.00 | Product Data Sheet |  |
| Active Enzyme | Cat. # | Size | Price (USD) | Data Sheet |
Add to Cart |
| Active Human Calpian I | 1134-100 | 10 ug | $235.00 | Product Data Sheet | |
| Inhibitor | Cat. # | Size | Price (USD) | Data Sheet |
Add to Cart |
| Calpain Inhibitor, Z-Leu-Leu-Tyr-FMK (10mM) | 1125-20C | 20 ul | $115.00 | Product Data Sheet | |
 Calpain Antibodies |
Cat. # | Size | Price (USD) | Data Sheet |
Add to Cart |
| Calpain 1 Polyclonal Antibody (WB) (hu) | 3189-100 | 100 ug | $225.00 | Product Data Sheet | |
| Calpain 2 Polyclonal Antibody (WB) (hum/ms/rt) | 3372-100 | 100 ul | $225.00 | Product Data Sheet | |
| Calpain 3 Polyclonal Antibody (WB) (hu) | 3373-100 | 100 ul | $225.00 | Product Data Sheet | |
| Calpain 5 Polyclonal Antibody (WB) (hu/ms/rt) | 3375-100 | 100 ul | $225.00 | Product Data Sheet | |
| Calpain 6 Polyclonal Antibody (WB) (hu/ms/rt) | 3376-100 | 100 ul | $225.00 | Product Data Sheet | |
| Calpain 7 Polyclonal Antibody (WB) (hu/ms/rt) | 3377-100 | 100 ul | $225.00 | Product Data Sheet | |
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 Additional Products |
Cat. # | Size | Price (USD) | Data Sheet |
Add to Cart |
| Calpain Inhibitor, Z-Leu-Leu-Tyr-FMK (10mM) | 1125-20C | 20 ul | $115.00 | Product Data Sheet | |
| Cathepsin B & L Inhibitor, Z-Phe-Phe-FMK (10mM) | 1126-20C | 20 ul | $155.00 | Product Data Sheet | |
| Granzyme B Inhibitor, Z-Ala-Ala-Asp-CMK (10mM) | 1128-20C | 20 ul | $125.00 | Product Data Sheet | |
| EMAP II Inhibitor, Z-ASTD-FMK | 1127-20C | 20 ul | $195.00 | Product Data Sheet | |
| Biotinylated Caspase-Family Inhibitor, Biotin-VAD-FMK | 1123-20C | 20 ul | $255.00 | Product Data Sheet | |
| Biotinylated Caspase-3 Inhibitor, Biotin-DEVD-FMK | 1124-20C | 20 ul | $295.00 | Product Data Sheet | |
| Biotinylated Caspase-8 Inhibitor, Biotin-DEVD-FMK | 1121-20C | 20 ul | $295.00 | Product Data Sheet | |
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