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Quick Detection of Apoptotic DNA Ladder in Jurkat Cells. Apoptosis was induced in Jurkat cells with camptothecin (2 uM) for 0 hr (Lane 1), 6 hrs (Lane 2) and 12 hrs (Lane 3). Chromosomal DNA was prepared using the Quick Apoptotic DNA Ladder Detection Kit according to the kit instructions. 20 ml of each sample was electrophoresed on a 1.2% agarose/EtBr gel.
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Detection of Apoptotic Cells Using Apo-BrdUTM Kit by Flow Cytometer. Apoptosis was induced in Jurkat cells with camptothecin (2 uM) for 8 hours. Control cells (uninduced) and induced cells were both collected, labeled with ApoBrdU according to the kit instructions. Results were analyzed by flow cytometry.
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