BioVision offers several novel kits for fast and sensitive detection of DNA ladder in apoptotic cells. Unlike other similar kits in the market which require 1-2 days to prepare nucleic acids, the new procedure requires less than 90 minutes to prepare chromosomal DNA, requiring neither extraction nor a column. The newly released Apoptotic DNA ladder extraction kit can detect apoptotic DNA ladder from tissue and cells that contain even 5% apoptotic cell population. DNA ladders can easily be visualized by agarose gel electrophoresis.

The TUNEL-based DNA fragmentation assay kits provide complete components including positive and negative control cells or tissue slides for convenient detection of DNA fragmentation in cultured cells and tissue sections. Results can be analyzed by flow cytometry or fluorescence microscopy.

Quick Detection of Apoptotic DNA Ladder in Jurkat Cells. Apoptosis was induced in Jurkat cells with camptothecin (2 uM) for 0 hr (Lane 1), 6 hrs (Lane 2) and 12 hrs (Lane 3). Chromosomal DNA was prepared using the Quick Apoptotic DNA Ladder Detection Kit according to the kit instructions. 20 ml of each sample was electrophoresed on a 1.2% agarose/EtBr gel.
Detection of Apoptotic Cells Using Apo-BrdUTM Kit by Flow Cytometer. Apoptosis was induced in Jurkat cells with camptothecin (2 uM) for 8 hours. Control cells (uninduced) and induced cells were both collected, labeled with ApoBrdU according to the kit instructions. Results were analyzed by flow cytometry.

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Quick Apoptotic DNA Ladder Detection Kit K120-50 50 assays $245.00 Product Data Sheet
Provides a simple procedure to prepare chromosomal DNA less than 90 minutes. DNA ladders can be visualized by agarose gel electrophoresis. The kit extracts both genomic DNA and laddered DNA.

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Enhanced Apoptotic DNA Ladder Detection Kit K130-50 50 assays $295.00 Product Data Sheet
Provides a simple procedure to prepare chromosomal DNA less than 90 minutes. DNA ladders can be visualized by agarose gel electrophoresis. The new procedure significantly improves sensitivity of detection with a unique DNA staining dye, provided in the kit.

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Apoptosis DNA Ladder Extraction Kit K170-50 50 assays $295.00 Product Data Sheet
Provides a new procedure for sensitive detection of Apoptotic DNA ladder. Apoptotic DNA ladder can be easily visualized by agarose gel electrophoreses. The kit specifically extracts laddered DNA, not genomic DNA. Therefore, the kit can detect apoptotic DNA ladder from both tissues and cells containing as little as 5% apoptotic cell population.

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ApoBrdU DNA Fragmentation Assay Kit K401-60 60 assays $495.00 Product Data Sheet
Provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells by flow cytometry.

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ApoBrdU-Red DNA Fragmentation Kit K404-60 60 assays $495.00 Product Data Sheet
Also provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells by flow cytometry. The assay is suitable for GFP transfected cells.

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ApoBrdU-IHC DNA Fragmentation Assay Kit K403-50 50 assays $565.00 Product Data Sheet
Provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation in tissue sections and fixed cell preps. Results can be analyzed by microscopy.

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ApoDIRECT DNA Fragmentation Assay Kit K402-50 50 assays $495.00 Product Data Sheet
Provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells by flow cytometry.

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Nuclear/Cytosol Fractionation Kit K266-100 100 assays $285.00 Product Data Sheet
Provides a complete system for separating nuclear fraction from the cytoplasm of mammalian cells and tissues. Extracted fractions are compatible with downstream assays.

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Genomic DNA Isolation Kit K281-50 50 assays $195.00 Product Data Sheet
Provides unique formulations of buffers and reagents for convenient isolation of highly pure genomic DNA.

Name
Antibodies to Nuclear Related Proteins

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