|Highlights||• Amount - 20 µg
• Application: C-terminal mammalian expression vector encoding humanized TagGFP and allowing TagGFP expression and generation of fusions to the TagGFP C-terminus.
Generation of VisionGFP-tagged fusions A localization signal or a gene of interest should be cloned into MCS of the vector. It will be expressed as a fusion to VisionGFP C-terminus when inserted in the same reading frame as VisionGFP and no in-frame stop codons are present. VisionGFP-tagged fusions retain fluorescent properties of the native protein allowing fusion localization in vivo. Unmodified pVisionGFP-C vector will express VisionGFP when transfected into eukaryotic (mammalian) cells.
Expression in Mammalian Cells- pVisionGFP-C can be transfected into mammalian cells by any known transfection method. CMV promoter provides strong, constitutive expression of VisionGFP or VisionGFP-tagged fusions in many cell types. If required, stable transformants can be selected using G418.
Propagation in E. coli
-Suitable host strains: DH5alpha, HB101, and other general purpose strains. Single-stranded DNA production requires a host containing an F plasmid such as JM109 or XL1-Blue.
- Selectable marker: plasmid confers resistance to kanamycin (30 µg/ml) to E. coli hosts.
-E. coli replication origin: pUC
- Copy number: ~500 -Plasmid incompatibility group: pMB1/ColE1
|USAGE||For Research Use Only! Not For Use in Humans.|