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Home » » Proteins and Enzymes » Proteins and Enzymes (A-Z) » 6501-1
Products and Applications 

Protein A Sepharose

Catalog# Size Price    
6501-1 1 ml $75.00 Add to Cart
6501-100100 ml$2295.00Add to Cart
6501-2525 ml$795.00Add to Cart
6501-55 ml$225.00Add to Cart

Contents:
Supplied as a 50% slurry in 20 % Ethanol/H2O.

Features:
High binding capacity = Binding of IgG ≥16 mg human or rabbit IgG/ml Protein A-Sepharose.
Minimal Leaching of the Ligand
Flow Rate Tested* = 2.07 ml/min.
*Test condition: = Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm).
Usage = Reusable for up to 10 times without significant loss of binding capacity.

Applications:
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.

Description:
Protein A-Sepharose beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Sepharose beads. BioVision Protein A (Cat. # 6500, Cat. # 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A. The IgG binding capacity of Protein A-Sepharose is ≥ 16 mg human or rabbit IgG per ml of wet beads. Protein A-Sepharose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.

Storage Temp.:
+4°C

Shipping:
gel pack

Usage: For Research Use Only! Not For Use in Humans.

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