Protease Inhibitor Cocktail :: 800-891-9699

Protease Inhibitor Cocktail. Crude cell extracts contain many endogeneous enzymes, such as proteases and phosphotases, which can degrade valuable proteins in the extract. The protease inhibitor cocktail contains a mixture of water-soluble protease inhibitors with broad specificity for various proteases, and has been optimized to generate the maximal protection to mammalian tissue and cell extracts.

Products	Cat. #	Size	Price (USD)	Data Sheet	Add to Cart
Protease Inhibitor Cocktail	K271-500	1 kit	$135.00	Product Data Sheet

I. Form: Supplied as a lyophilized powder

II. Package Quantity: One vial can make 125 ml of cocktail solution.

III. Preparations: Add 250-ul DMSO to the vial, pipet several times to dissolve all powder (This makes 500X concentrated inhibitor cocktail).

IV. Components:

Component

Concentration (w/v)

Aprotinin

Leupeptin

Pepstain A

PMSF

0.1%

0.035%

8.5%

V. Product Profile:

The individual components of the protease inhibitor cocktail have specific inhibitory properties. A description of each inhibitor is given below:

Aprotinin: Inhibits serine proteases, such as trypsin, chymotrypsin, plasmin, trypsinogen, urokinase, and kallikrein.

Leupeptin: Inhibits both serine and cysteine proteases, such as calpain, trypsin, papain, and cathepsin B.

Pepstain A: Inhibits acid proteases, such as pepsin, rennin, cathepsin D, chymosin, and protease B.

PMSF: Inhibits serine proteases, such as trypsin and chymotrypsin. Also inhibits cysteine proteases and mammalian acetylcholinesterase.

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