DNA Ligation Products

BioVision offers numerous DNA Ligation Products in the Molecular Biology portfolio. This includes the 5 Minutes DNA Ligation Kit, T4 DNA Ligase, T4 RNA Ligases and E. coli DNA Ligase. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. E. coli DNA Ligase is a NAD+-dependent enzyme and an alternative to T4 DNA Ligase when blunt-end ligation is not required and is used for cDNA cloning of products from second strand cDNA synthesis experiments. T4 RNA Ligase 1 (ssRNA Ligase) are for the ligation of a ssRNA and DNA or for the synthesis of single-stranded oligonucleotides. T4 RNA Ligase 2 (dsRNA Ligase) ligates nicks in dsRNA and is suitable for ligating the 3' OH terminus of RNA to the 5' phosphate terminus of DNA to form a DNA/RNA hybrid. T4 RNA Ligase 2 (truncated) is a double-point mutant of T4 RNA Ligase 2 which specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Key Features:

  • • Ready-To-Use Reagents
  • • Fast and Convenient
  • • Free of Contaminating Exonucleases and Endonucleases
  • • For Both Sticky-End or Blunt-End DNA Fragments (Nick repair of duplex DNA, RNA or DNA-RNA hybrids etc)
  • • Many Downstream Applications: Nick repair of duplex DNA, RNA or DNA-RNA hybrids; Self-circularization of linear DNA; Site-directed mutagenesis; Cloning of restriction fragments; ligation of a ssRNA and DNA; ligate nicks in dsRNA etc

DNA Ligation Product List

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2 Item(s)

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