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Glycogen Assay Kit. Glycogen is the primary short term energy storage molecule in animals. It is synthesized primarily in the liver and muscle. Glycogen is a highly branched polymer of glucose molecules, connected with an α-1,4 linkage, branching via an α-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases. We have developed an easy, and convenient assay to measure glycogen level in biological samples. In the assay, glucoamylase hydrolyzes the glycogen to generate glucose which is then oxidized to generate a products that reacts with OxiRed probe to generate color ( λmax = 570 nm) and fluorescence ( Ex 535/Em 587). The assay can detect glycogen concentration 0.0004 to 2 mg/ml.
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