Phosphate Colorimetric Assay Kit

SKU: K410

Availability: In stock



Phosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use. The newly designed Phosphate Colorimetric Assay Kit provides an easy, quick and sensitive means of assessing phosphate over a wide range of concentrations. The assay utilizes a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around 650 nm. Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined. The Phosphate Colorimetric Assay Kit provides 500 assays using microtiter plates or 100 assays using 1 ml cuvettes.

Product Details

Size 500 assays
SKU+Size K410-500
Detection Method Absorbance (650 nm)
Species Reactivity Mammalian
Applications Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
Features & Benefits • Simple procedure; takes ~ 3 hours
• Fast and convenient
• The assay is sensitive and stable
• The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Kit Components • Phosphate Reagent • Phosphate Standard (10 mM)
Storage Conditions RT
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.
What are the possible sources of interferences for this assay?
1. We ask our customers to avoid using glasswares since a lot of laboratory detergents contain high amounts of phosphate. Disposable plastic wares are therefore recommended for the assay and sample preparation. 2. Presence of IgG and IgM paraproteins can produce false increase in Phosphate concentration by producing precipitates in the reaction. Dilution of the sample can reduce the interference. Deproteination of the samples works best (Clin Chem. 1995 Apr;41(4):60914.). 3. It’s advisable to avoid TritonX during sample preparation4. Some reports suggests Trichloroacetic acid and Tungtic acid can interfere with the assay5. EDTA, oxalate and citrate should be avoided6. Significant interference can be caused by hemolysate, bilirubin and intralipids.