100 assays. This Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells and tissues. The new kit provides a system that maintains the nuclear and cytoplasmic compartments separate and intact. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic extracts from cells relatively quick with little or no cross-contaminations. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with downstream assays such as transcriptional activity/reporter assays/enzyme activity assays/Western blotting and more.

 

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