Luciferase Antibody

Catalog #: 3848

Availability: In stock

$245.00

Product Details

Cat # +Size 3848-500
Size 500 µg
Antibody Target Luciferase
Host Goat
Antibody Type Polyclonal
Isotype Goat IgG
Immunogen Recombinant Firefly luciferase
Appearance Colorless liquid
Concentration 10.0mg/ml
Formulation 500 µg (10 mg/ml) goat anti-Luciferase (firefly) polyclonal antibody in 0.02 M potassium Phosphate and 0.15 M sodium chloride, pH 7.2.
Purification Affinity purified
Species Reactivity Luciferase
Application Western blot, Immunohistochemistry, ELISA
Application & Usage Western blotting (1:5000-20,000), ELISA (1:50,000-200,000), and Immunohistochemistry (1:1000-1:5000). However, the optimal conditions should be determined individually. The antibody recognizes firefly luciferase. It does not react with Renilla luciferase.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

The Firefly luciferase is a genetically modified variant derived from the Luciferase of Diaphanes pectinealis endemic to Yunnan province, China. It has been modified to provide enhanced stability compared to the normal phenotype of Photinus pyralis as well as a broader and more physiologically relevant effective pH range. Luciferase has been used as a sensitive reporter for studying gene regulation and function. Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction.
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