Antibody Target: LOX-1
Alternative Name: LOK, Lymphocyte Oriented Kinase, STK10, STK-10, Serine/Threonine Kinase 10
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide near the N-terminus of human Lox-1
Gene ID: 4973
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-LOX-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat
Positive Control: Western Blot: Jurkat cell lysate
Application: Western blot, Immunohistochemistry
Application & Usage: The antibody can be used for Western blotting (0.5-4 µg/ml), and in Immunohistochemistry (5 µg/ml) for detecting the pro- (~50 kDa) and the mature (~30 kDa) forms of Lox-1 in samples from human, mouse and rat origins. Other applications have not been determined. Blocking peptide (Cat. # 3659RBP-50) is available separately.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1), also known as oxidized low-density-lipoprotein receptor-1 (OLR-1), belongs to the C-type lectin family. LOX-1 binds and supports the internalization of multiple structurally unrelated macromolecules including oxidized LDL, advanced glycation end products (AGE), activated platelets, bacteria, apoptotic or aged cells, and heat shock proteins. Human LOX-1 gene encodes a 273 amino acid residues (aa) protein with a short N-terminal intracellular domain, a transmembrane domain, an extracellular stalk/neck region followed a C-type lectin-like domain (CTLD). The expression of LOX-1 is induced by proinflammatory or proatherogenic stimuli, as well as by oxidized LDL itself and hemodynamic or oxidative stress. LOX-1-dependent oxidized LDL uptake also induces apoptosis by inducing the expression of the pro-apoptotic Bax and downregulation of the anti-apoptotic Bcl-2.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans.