Alternative Name: BIRC7, LIVIN, ML-IAP , MLIAP, KIAP , RNF50 , Livin

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Immunogen: Synthetic peptide surrounding amino acid near the C-terminus of the long form of human Livin

Accession#: Q96CA5

Gene ID: 79444

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-Livin polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: human

Application: Western blot, Immunohistochemistry

Application & Usage: Western blotting (2-6 µg/ml). However, the optimal dilution conditions should be determined individually. The antibody detects a band at ~33 kDa and a weaker band at 30 kDa, corresponding to Livin α and β. Raji cell lysate can be used as a positive control.

Storage Temp.: -20 °C

Shipping: gel pack

Background Descriptions: Livin is a novel member in the IAP (inhibitor of apoptosis) protein family. Livin contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin-α and Livin-β. Transfection of Livin protected cells from apoptosis induced by FADD, BAX, RIP, RIP3 and DR6. Livin directly reacts with several caspases including Caspase-3, -7, and –9, and it inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c and ATP.

Handling: The antibody solution should be gently mixed before use.

Usage: For Research Use Only! Not to be used in humans.