Alternative Name: IRS1 , HIRS-1

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Immunogen: Synthetic peptide mapping to the C-terminus of human IRS-1

Accession#: P35568

Gene ID: 3667

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: human, mouse, rat

Application: Western blot, Immunohistochemistry

Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually

Storage Temp.: -20 °C

Shipping: gel pack

Background Descriptions: IRS-1 (Insulin receptor substrate-1) is phosphorylated in response to stimulation of cells by insulin. Upon ligand-induced phosphorylation, IRS-1 immediately associates with a series of SH2 domain-containing signaling intermediates such as PI3-kinase, GRB2, Syp and Nck. The level of diversity in structure and function of the SH2-containing proteins which binds activated IRS-1 is important for responsiveness to insulin. IRS phosphorylation elicits the growth and metablic effects of insulin growth factors and insulin by allowing glucose uptake, glycogen synthesis and mitogenic activity. IRS-1 migrates between 170 and 185 kDa and is expressed in most cell types.

Handling: The antibody solution should be gently mixed before use.

Usage: For Research Use Only! Not to be used in humans.