100 assays. Iron is essential to nearly all known organisms. BioVision provides a simple, convenient assay kit to measure Ferrous (Fe2+) or Ferric (Fe3+) ion in various samples. In the assay, ferric carrier protein will dissociate ferric into solution in the presence of acid buffer. After reduction to the ferrous form (Fe2+), iron reacts with Ferene S to produce a stable colored complex and give absorbance at 593 nm. A specific chelate chemical is included in the buffer to block copper ion (Cu2+) interference. The kit measures iron in the linear range of 0.4 to 20 nmol in 50 ul sample, or 8 uM to 400 uM iron concentration in various samples.

 

Iron assay, Iron assay kit, Iron analysis, iron detection