Alternate Names / Synonyms: IL27, Interleukin27, IL 27, interleukin 27

Gene Symbol: IL27

Accession #: Q8K3I6

Gene ID: 246779

Source: E. coli

Appearance: Lyophilized protein

Physical Form Description: Lyophilized from 10 mM NaP, pH 7.5

Molecular Weight: 23.7 kDa

Purity by SDS-PAGE: ≥97%

Purity by HPLC: ≥97%

Endotoxin Level: <0.1 ng/μg of IL-27

Biological Activity: Measured by dose-dependent inhibition of TGF-β & Il-6-induced IL-17A expression in mouse CD4 splenocytes. 50 ng/ml of mouse p28 is capable of inhibiting ≥25% of Il-17A expression in this assay.

Reconstitution Instructions: Reconstitute in water to a concentration of 0.1-0.5 mg/ml. The solution can then be diluted into other aqueous buffers

Storage Temp.: -20°C

Shipping: Gel Pack

Background Information: The p28 subunit of IL-27 (IL-27/p28), also known as Interleukin-30 (IL-30), is a member of the IL-12 family of cytokines.  When combined with EBI3 (Epstein-Barr virus induced gene 3), p28 forms a heterodimer known as IL-27.  p28 is thought to be a pro-inflammatory cytokine inducing immuno-modulatory effects, but the details of these effects are currently unclear and research is focused on delineating its specific biological functions.  Recombinant mouse p28 is a non-glycosylated protein, containing 207 amino acids, with a molecular weight of 23.7 kDa.

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans.