Alternate Names / Synonyms: human flt3-Ligand, flt3-Ligand, flt3Ligand, flt3 Ligand,h-flt3-Ligand, rh-flt3-Ligand, recombinant human flt3-Ligand, recombinant flt3-Ligand
Gene Symbol: Flt3-L
Accession #: P49771
Gene ID: 2323
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered and lyophilized with no additives. The protein may appear as a haze of film, which is difficμlt to see at the bottom of the vial.
Molecular Weight: 17.6 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of human AML5 cells is <1.0 ng/ml, corresponding to a specific activity of > 1 x 10⁶ units/mg.
Reconstitution Instructions: Reconstitute in water to a concentration of 0.1 – 1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Human flt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells. Recombinant human flt3-ligand is a soluble 17.6 kDa polypeptide containing 155 amino acid residues, which comprises the extraCellular domain of the transmembrane flt3-ligand protein.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.