EZEnrich™ Polyubiquitin Beads
Binding Capacity: ~ 2.06 µg/20 µl of slurry.
Bead content: 300 µl of 50% slurry of UIM-UBA Sepharose Beads in PBS with 0.1% Sodium Azide.
Total Capacity: 15 enrichments with 20 µl beads each.
Specificity: The EZEnrich™ Polyubiquitin Beads bind polymers of ubiquitin containing at least 4 ubiquitin subunits.
• Isolation/enrichment of poly-ubiquitinated proteins from complex samples including tissue homogenates, whole cell lysates, subcellular organelles like mitochondria, endoplasmic reticulum, etc, for multiple downstream applications like proteomic studies, protein ubiquitination status tests etc.
• Recovery of poly-ubiquitinated proteins from in vitro ubiquitination reactions.
• Subjective quantitation of the differences in the ubiquitination of different target proteins.
BioVision’s EZEnrich™ Polyubiquitin Beads are ideal for isolating or enriching polyubiquitin modified proteins from biological samples. Ubiquitin is a highly conserved 76-amino acid protein. It can be conjugated via its C-terminus to the amine groups of lysine residues on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. Different types of polyubiquitin chains can form, depending on the internal lysine residue used for this conjugation. These polyubiquitin chains further can attach to proteins post-translationally and aid in numerous downstream activities like proteasome-mediated proteolysis, autophagy, DNA damage tolerance, inflammation, apoptosis, signal transduction etc. Several classes of Ubiquitin interacting proteins help in mediating these downstream effects. Ubiquitin Interacting Motifs (UIM) and Ubiquitin Associated Domains (UBA) are two large classes of such protein domains which strongly interact with polyubiquitin chains. We have developed an UIM-UBA chimeric protein from the UIM and UBA domains. Both these proteins are well-characterized for their high-affinity interaction with different types of polyubiquitin chains. The UIM-UBA protein is covalently bound to our sepharose beads (Cat # 6565) leading to a high binding affinity. These UIM-UBA sepharose beads are ideal for convenient and efficient enrichment of poly-ubiquitinated proteins, with minimal contamination.
Usage: For Research Use Only! Not For Use in Humans.