The one-step staining procedure takes only 10 minutes. Annexin V-FITC conjugate is specially engineered to produce enhanced fluorescence signal and photo stability. The Annexin V-FITC kit includes Annexin V-FITC for detecting apoptosis and also propidium iodide (PI) for detecting necrosis. Thus, Apoptosis and necrosis can be differentiated. Detection can be analyzed by flow cytometry (Ex/Em=488nm/530nm) or by fluorescence microscopy using a dual filter set for FITC and rhodamine. The Annexin VFITC Plus kit includes cytox green dye (instead of PI) which stains necrotic cells with a higher level of green fluorescence than FITC does. Thus, both apoptosis and necrosis can be detected using FL1 channel.