BioVision offers unique recombinant protein A, G, L. These genetically engineered proteins contain only the IgG binding regions and have 6X His-tag on their N-terminus. Other domains such as cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from all these three proteins to ensure the maximum specific IgG binding. The 6X His-tag on N-terminus of these proteins can be used for affinity purification or for protein A/G/L detection using anti-His-tag antibody (Cat.# 3998-100). These unique, high quality recombinant Protein A, G and L are produced in E. coli, and purified using multiple chromatographic steps (not affinity purified with human IgG). Purity is > 98% by SDS-PAGE and HPLC. Endotoxin level is < 0.05 EU/mg of Protein. Protein A, G and L bind to most mammalian immunoglobulins in their Fc binding domains and therefore are ideal for purification of polyclonal or monoclonal IgG antibodies. The combinations of Protein A/G and Protein A/G/L are also available, allowing the maximum recovery during antibody purifications.
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