|Detection Method||Flow cytometry (Ex = 488 nm; Em = 670 nm) and fluorescence microscopy|
|Applications||Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.|
|Features & Benefits||• Simple one-step procedure; takes only 10 minutes
• Fast and convenient, no need of fixation of cells
|Kit Components||• Annexin V-PE-Cy5
• 1X Binding Buffer
|Shipping Conditions||Gel Pack|
|USAGE||For Research Use Only! Not For Use in Humans.|
Can I do labeling with another antibody post Annexin-V labeling? If I permeabilize after fixation and do another antibody labeling then my annexin staining disappears. Does this have to do with the fluorophore or the permeabilization step?
Can the incubation with Annexin V be extended for 30 m in at RT?
I am interested in the kit for high throuphput screening. Can we use a plate reader to detect Annexin V?
For this kit, where was the Annexin-V obtained from? Is it recombinant? Or purified? And from what species?
Can the kit work on bacteria or yeast cells?
What is the exact volume of sample required for this assay?
Do you have trial sizes of this kit?
What is the shelf life of this kit?
Can we purchase individual components of this kit?
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?