|Detection Method||Flow cytometry (Ex = 543 nm; Em = 570 nm) and fluorescence microscopy|
|Features & Benefits||• Simple one step staining procedure in 10 minutes
• Fast and convenient
• Cy3 shows brighter red fluorescence
|Kit Components||• Annexin V-Cy3
• 1X Binding Buffer
|Shipping Conditions||Gel Pack|
|USAGE||For Research Use Only! Not For Use in Humans.|
The protocol in the datasheet for flow cytometry doesnt mention a wash step after Annexin/PI addition. can i confirm that this is correct?? I.e, 5min incubation then straight into flow cytometer
Once your cells have been re-suspended in PBS, you can also add a preservative/stabiliser if you are planning on keeping your samples for a long time. For example, 1 mg/ml BSA or 1% sodium azide.
I am looking to investigate cell apoptosis using 96 well plates. I was wondering if your Annexin V-FITC Apoptosis Detection Kit could be adapted to a 96 well plate fluorescent plate reader format?
Can you prelabel the cells and then follow apoptosis using this kit?
For this kit, where was the Annexin-V obtained from? Is it recombinant? Or purified? And from what species?
Can the kit work on bacteria or yeast cells?
What is the exact volume of sample required for this assay?
Do you have trial sizes of this kit?
What is the shelf life of this kit?
Can we purchase individual components of this kit?
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Will trypsinizing the cells removed the phosphatidylserine?